Journal: International Dental Journal

Article Title: MZB1 Drives Periodontitis via ER Stress-Mediated Inflammation and Osteogenic Differentiation

doi: 10.1016/j.identj.2026.109452

Figure Lengend Snippet: Knockdown of MZB1 restores osteogenic differentiation capacity of PDLSCs under inflammatory conditions (A) Microscopic images of alizarin red S staining showing calcium nodule formation after osteogenic induction of PDLSCs under different conditions. (B) Quantification of alizarin red S staining by absorbance at 560 nm (N = 3). (C) Quantitative analysis of alkaline phosphatase (ALP) activity following osteogenic induction of PDLSCs (N = 3). (D) Western blot images of osteogenic differentiation-related proteins (RUNX2, BMP2, α-SMA, and Collagen I) in PDLSCs. (E) Quantification of Western blot bands normalised to β-Actin, representing relative expression levels of osteogenic markers (N = 3).

Article Snippet: After determining protein concentrations, ALP activity was measured using an ALP activity assay kit (Nanjing Jiancheng Bioengineering Institute, China).

Techniques: Knockdown, Staining, Activity Assay, Western Blot, Expressing

Journal: Vaccines

Article Title: Cellular Immune Response and T Cell Epitope Mapping of Plasmodium falciparum Chimeric Vaccine Candidate GMZ2.6c and Its Components (MSP-3, GLURP and Pfs48/45) in Individuals Naturally Exposed to Malaria in Brazilian Amazon

doi: 10.3390/vaccines14050423

Figure Lengend Snippet: IFN-γ responses to MSP-3, GLURP, and Pfs48/45 synthetic peptides. Frequency of positive responders ( A ) and numbers of spots-forming units (SFU) ( B ) of IFN-γ responses to Mp 1 (blue), Ppp I (green), Ppp II (gray), and Gpp I (red) in exposed P. falciparum -infected (PFI, n = 20), non-infected (NI, n = 25), and non-endemic control (Control, n = 10) individuals. Bars represent the frequency of responders ( A ) and medians of adjusted SFU of IFN-γ responses to each peptide pool ( B ). Lines represent the interquartile ranges. Dashed red lines represent the positivity limit. Statistical significance was calculated using chi-square, Mann–Whitney test, and Kruskal–Wallis test, followed by Dunn’s post hoc test. Significant differences are indicated by * (* p < 0.05; *** p < 0.0005).

Article Snippet: ELISpot assays were carried out using the commercial kit ELISpot Plus: Human IFN-γ (ALP) (MabTech, Nacka Strand, Sweden) according to the manufacturer’s instructions.

Techniques: Infection, Control, MANN-WHITNEY